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表观遗传变异是体细胞无性系变异产生的重要基础。为研究红掌体细胞突变过程中DNA甲基化的变异,本研究采用MSAP(methylation-sensitive amplification polymorphism)技术对红掌品种‘阿拉巴马’(Anthurium andraeanum Lind.‘Alabama’)正常株及白色佛焰苞变异株基因组胞嘧啶的甲基化模式进行了比较和分析。121对引物共扩增出5 109条带,红掌正常株和变异株基因组中分别检测到1 834和1 859个位点的DNA胞嘧啶发生甲基化,甲基化率为36.37%和36.70%,其中,胞嘧啶内侧全甲基化的发生频率高于外侧半甲基化。正常株与变异株间有209个位点(4.09%)的甲基化状态存在变异,主要分为4组变化类型,变异模式以重新甲基化和完全去甲基化为主,分别占多态性甲基化位点的34.45%和31.58%。研究结果显示,红掌‘阿拉巴马’正常株与变异株间基因组胞嘧啶甲基化状态存在较大差异,组织培养过程中产生的佛焰苞表型的变异可能与其基因组甲基化模式的改变有关。
Epigenetic variation is an important basis for somatic clonal variation. In order to study the variation of DNA methylation during the process of somaclonal variation in Anthurium andraeanum Lind.’Alabama ’, we used MSAP (methylation-sensitive amplification polymorphism) The methylation patterns of cytosine in the genome of spathe variant were compared and analyzed. A total of 109 bands were amplified with 121 pairs of primers. DNA methylation of 1 834 and 1 859 DNA methylation sites was found in the normal and variant strains of Anthurium, with the methylation rates of 36.37% and 36.70 %, Of which, cytosine medial full-methylation occurred more frequently than the lateral hemomethylation. The methylation status of 209 loci (4.09%) between the normal strains and the mutants varied. They were mainly divided into four groups and the patterns of variation were mainly remethylation and complete demethylation 34.45% and 31.58% of the state methylation sites. The results showed that there was a big difference in genomic cytosine methylation status between the normal and mutant strains of Anthurium andraean, and the variation of the phenotype of the spathe in tissue culture may be related to the genomic methylation pattern Change related.