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取Wistar大鼠36只,按体重随机分成3组,每组12只。第1～3组分别为对照组、低甲基汞(MeHg)组和高MeHg组。每日染毒1次,连续4周。每周测量大鼠体重,用冷原子吸收法测量大鼠脑皮质内Hg含量,比色法测定GS活力、谷氨酸(Glu)和谷氨酰胺(Gln)含量,用Real-time PCR和Western blotting检测GS mRNA表达和蛋白水平。与对照组比较,在低MeHg组中Hg含量明显升高,GS活力、GS蛋白水平均显著下降;在高MeHg组中,大鼠体重、GS活力、Gln含量、GSmRNA表达和蛋白水平均明显下降,Hg和Glu含量均显著增高。提示甲基汞能导致大鼠脑谷氨酰胺合成酶表达和功能异常,谷氨酰胺合成酶在甲基汞诱导的神经毒性过程中起重要作用。
Thirty-six Wistar rats were randomly divided into three groups according to body weight, with 12 rats in each group. Groups 1 ~ 3 were control group, MeHg group and MeHg group respectively. Daily poisoning 1, for 4 weeks. The body weight of rats was measured every week. The content of Hg in rat cerebral cortex was measured by cold atomic absorption spectrometry. The contents of GS, Glu and Gln were determined by colorimetric assay. Real-time PCR and Western The levels of GS mRNA and protein were detected by Western blotting. Compared with the control group, the content of Hg in the low MeHg group was significantly increased, the GS activity and the GS protein level were significantly decreased. In the high MeHg group, the body weight, GS activity, Gln content, GS mRNA expression and protein level were significantly decreased , Hg and Glu content were significantly increased. It is suggested that methylmercury can induce the expression and dysfunction of glutamine synthetase in rat brain. Glutamine synthetase plays an important role in methylmercury-induced neurotoxicity.