Real time detection of antibody-antigen interaction using a laser scanning confocal imaging-surface

• 作 者: 张洪艳 杨立泉 孟岚 聂家财 宁廷银 刘卫敏 孙嘉宇 汪鹏飞
• 机 构: Key Laboratory of Photochemical Conversion and Optoelectronic Materials,Technical Institute of Physi
• 出 处: Chinese Physics B
• 发表日期: 2012年02期
• 关键词: plasmon confocal biosensor instrument resonant affinity bonding labeled IgG citr

A laser scanning confocal imaging-surface plasmon resonance (LSCI-SPR) instrument integrated with a wavelength-dependent surface plasmon resonance (SPR) sensor and a laser scanning confocal microscopy (LSCM) is built to detect the bonding process of human IgG and fluorescent-labeled affinity purified antibodies in real time.The shifts of resonant wavelength at different reaction time stages are obtained by SPR,corresponding well with the changes of the fluorescence intensity collected by using LSCM.The instrument shows the merits of the combination and complementation of the SPR and LSCM,with such advantages as quantificational analysis,high spatial resolution and real time monitor,which are of great importance for practical applications in biosensor and life science.

A laser scanning confocal imaging-surface plasmon resonance (LSCI-SPR) instrument integrated with a wavelength-dependent surface plasmon resonance (SPR) sensor and a laser scanning confocal microscopy (LSCM) is built to detect the bonding process of human IgG and fluorescent- labeled affinity purified antibodies in real time. The shifts of resonant wavelength at different reaction time stages are obtained by SPR, corresponding well with the changes of the fluorescence intensity collected by using LSCM. The instrument shows the merits of the combination and complementation of the SPR and LSCM, with such advantages as quantificational analysis, high spatial resolution and real time monitor, which are of great importance for practical applications in biosensor and life science.