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煮沸法从粪便标本中快速抽提出细菌DNA作为聚合酶链反应(PCR)模板,用自行设计的1对特异性引物扩增位于志贺菌和侵袭性大肠埃希菌染色体和质粒DNA上的多拷贝侵袭性质粒抗原(Ipa)H基因,扩增产物为620bp,以常作者单位:310003杭州,浙江医科...
Boiling method was used to extract bacterial DNA from stool samples rapidly as polymerase chain reaction (PCR) template. A pair of specific primers designed by ourselves was used to amplify the DNA located on chromosome and plasmid DNA of Shigella and Escherichia coli Copy of invasive plasmid antigen (Ipa) H gene, the amplified product was 620bp, often the author: 310003 Hangzhou, Zhejiang Medical ...