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用氮离子、碳离子不同能量、剂量组合注入4个甜菊品种的干种子,经田间栽培、收获时测定产量及糖甙含量变化,结果如下:1)同一品种(济宁种)不同处理,糖甙产量差异均达显著平准。其中以75keV、5×1014/cm2氮离子注入组合最佳,公顷产干叶、总甙、St甙、RA甙产量分别比对照提高15%、5%、3%及14%。2)能量、剂量及离子作用比较,能量作用以60keV可促进干叶产量提高;75keV可促进糖甙产量的提高。剂量作用没有一定规律。初步看出5×1014对干叶产量及糖甙产量有促进作用。离子作用看出,氮离子可促进干叶产量提高,碳离子可促进糖甙产量的提高。3)4个参试品种比,以纯品种表现趋势较一致,杂交种变异大,如日原×丰2品种75keV×1014碳离子注入组,干叶产量、总甙、St甙、RA甙及RC甙产量分别比对照高23%、42%、39%、28%及43%,是个很有希望的育种材料。
The dry seeds of four Stevia cultivars were infused with different energy and dose of nitrogen ions and carbon ions. The yield and contents of glucoside were determined after harvest. The results were as follows: 1) Different treatments of the same species (Jining species) Yield differences have reached a significant level. Among them, 75keV, 5 × 1014 / cm2 nitrogen ion implantation combination is the best. The yield of dry leaves, total glycosides, St glycosides and RA glycosides increased by 15%, 5%, 3% and 14%, respectively. 2) Compared with the energy, dose and ion effect, the energy of 60keV can promote the increase of dry leaf yield; 75keV can promote the increase of glycoside yield. Dosage does not have certain rules. The results showed that 5 × 1014 could promote the yield of dry leaves and the yield of glycoside. Ion effect shows that nitrogen ions can promote increased yield of dry leaves, carbon ions can promote the increase of glycoside yield. 3) Compared with the 4 tested varieties, the performance trend of the pure breeds was more consistent and the variation of hybrids was larger. For example, 75keV × 1014 carbon ion implantation group, dry leaf yield, total glycoside, St glycoside, RA glycoside And R C glycoside production were 23% higher than the control, 42%, 39%, 28% and 43%, is a promising breeding material.