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目的：探讨红藻氨酸（ Ｋ Ａ）诱导大鼠癫痫发作时海马ｍ Ｇｌｕ Ｒ１ ｍ Ｒ Ｎ Ａ 表达变化的时空特征． 方法：采用地高辛标记的ｃ Ｄ Ｎ Ａ 探针原位杂交法检测海马ｍ Ｇｌｕ Ｒ１ ｍ Ｒ Ｎ Ａ 表达，通过图像分析系统进行定量处理． 同时观察大鼠行为学及脑电变化． 结果： Ｋ Ａ 注射后大鼠均出现严重边缘性惊厥，脑电图表现为持续性癫痫样放电． 海马ｍ Ｇｌｕ Ｒ１ ｍ Ｒ Ｎ Ａ 表达在注射 Ｋ Ａ 后１ ｈ 开始增高，４ ｈ～８ ｈ 达高峰，之后逐渐回落，７２ ｈ降至最低． 结论： 在癫痫发作早期，ｍ Ｇｌｕ Ｒ１ 表达增强，提示其可能参与癫痫发作，并可能与发作后脑损害有关．
OBJECTIVE: To investigate the spatio-temporal characteristics of the expression of Glu R1 m R N A in the hippocampus of kainic acid (KA) -induced seizures in rats. Methods: The expression of GluR1 m R N A in hippocampus was detected by digoxigenin labeled c D N A probe in situ hybridization and quantified by image analysis system. At the same time observe the behavioral and EEG changes. RESULTS: Severe fronto-seizures were observed in rats after K A injection. EEG showed continuous epileptiform discharges. The expression of Glu R1 m R N A in hippocampus began to increase at 1 h after injection of K A and peaked at 4 h to 8 h, then gradually decreased and then decreased to the lowest at 72 h. Conclusion: In the early stage of epileptic seizure, the expression of m-Glu R1 is enhanced, suggesting that it may participate in seizures and may be related to brain damage after the onset of seizures.