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本研究组已经报道过缺碘地区甲状腺癌的组织学特征和P~(53)蛋白过表达之间的关系。为进一步明确本地区的少基因变异特点,我们对P~(53)蛋白过表达比较集中的甲状腺低分化癌标本的PCR产物进行单链构象多态性(SSCP)分析,结果为15例低分化癌中有14例P~(53)染色呈阳性,其阳性率为93.3％。P~(53)基因变异共在7例中被检出,检出率为46.7％。变异最多发生于外显子5中。结果显示P~(53)基因变异与甲状腺低分化癌密切相关,并且提示发生变异的甲状腺乳头状癌的恶性程度偏高。
The research team has reported the relationship between the histological features of thyroid cancer and the overexpression of P53 protein in iodine-deficient areas. To further clarify the characteristics of genetic mutations in this region, we analyzed the single-stranded conformation polymorphism (SSCP) of the PCR product of poorly differentiated thyroid carcinoma specimens with over-expressed P53 protein, resulting in 15 cases of poorly differentiated In cancer, 14 cases of P53 staining were positive, and the positive rate was 93.3%. P53 mutations were detected in 7 cases, and the detection rate was 46.7%. The variation occurred most in exon 5. The results showed that the P53 mutation was closely related to thyroid poorly differentiated carcinoma, and it indicated that the malignant thyroid papillary carcinoma with mutation had a high degree of malignancy.