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目的:探讨喉咽鳞状细胞癌组织中细胞角蛋白13(CK13)和细胞角蛋白4(CK4)的表达与喉咽鳞状细胞癌临床病理特征的关系。方法:应用免疫组织化学方法检测56例喉咽鳞状细胞癌患者的癌组织标本及癌旁正常组织中CK13及CK4的蛋白表达,应用VIDAS图像分析仪分析其图像的积分光密度,并进行统计学处理。结果:CK4与CK13在癌旁正常组织中均呈强阳性表达,而在喉咽鳞状细胞癌组织中的表达下降(P<0.01),且与病理分化程度均呈正相关(P<0.05)。CK4在T1、T2期的表达最强,在T3、T4期的表达依次减弱(F=93.814,P<0.05);CK4在Ⅰ~Ⅱ期的表达最强,在Ⅲ期、Ⅳ期中的表达逐渐减弱(F=127.894,P<0.05);CK4在无淋巴结转移的鳞状细胞癌组织中的表达较强,而在有淋巴结转移的鳞状细胞癌组织中表达较弱(T=10.678,P<0.05)。而CK13与肿瘤大小、临床分期及有无淋巴结转移无显著相关。结论:CK4与CK13共同检测对喉咽鳞状细胞癌的早期诊断、手术方法的选择以及判断预后具有指导意义。
Objective: To investigate the relationship between the expression of cytokeratin 13 (CK13) and cytokeratin 4 (CK4) in squamous cell carcinoma of the larynx and the clinicopathological features of squamous cell carcinoma of the larynx. Methods: Immunohistochemistry was used to detect the protein expression of CK13 and CK4 in 56 specimens of squamous cell carcinoma of laryngeal squamous cell carcinoma and adjacent normal tissues. The integral optical density of the specimens was analyzed by VIDAS image analyzer. Learn to deal with. Results: CK4 and CK13 were strongly positive in adjacent normal tissues and decreased in squamous cell carcinoma of the hypopharynx (P <0.01), and positively correlated with the degree of pathological differentiation (P <0.05). The expression of CK4 was the strongest at T1 and T2, and decreased at T3 and T4 (F = 93.814, P <0.05). The expression of CK4 at stage Ⅰ ~ Ⅱ was the strongest, while at stage Ⅲ and Ⅳ (F = 127.894, P <0.05). CK4 was strongly expressed in squamous cell carcinoma without lymph node metastasis and weakly in squamous cell carcinoma with lymph node metastasis (T = 10.678, P < 0.05). The CK13 and tumor size, clinical stage and with or without lymph node metastasis was not significantly correlated. Conclusion: The detection of CK4 and CK13 is helpful for the early diagnosis of hypopharyngeal squamous cell carcinoma, the selection of surgical methods and prognosis.