论文部分内容阅读
薇甘菊颈盲蝽Pachypeltis micranthus Mu et Liu(Hemiptera:Miridae)为世界性恶性杂草薇甘菊Mikania micrantha的自然天敌。在该天敌取食寄主的过程中,存在于其唾液中的多聚半乳糖醛酸酶作为重要的果胶水解酶在降解寄主植物细胞壁和引起寄主植物组织损伤中起着重要作用。然而,至今未有关于该天敌的任何基因信息被报道,本研究旨在获得其转录组数据,并基于该数据鉴定获得多聚半乳糖醛酸酶基因。采用Illumina测序技术进行转录组测定,共获得约5.2 Gbps的数据量,拼接得到57 739条非冗余基因(unigene)。通过BlastX注释,23 549条非冗余基因能与NCBI(National Center for Biotechnology Information)非冗余蛋白数据库中的已知蛋白相匹配,7 048、8 548和16 135条非冗余基因分别被成功的注释到GO(Gene Orthology)、COG(Clustes of Orthologous Groups)和KEGG(Kyoto Encyclopedia of Genes and Genomes)数据库中。基于构建的转录组数据库,共鉴定获得了24个多聚半乳糖醛酸酶基因,其中15个基因具有完整的开放阅读框。该转录组数据可为今后从分子水平研进一步研究薇甘菊颈盲蝽的生态学、生物学和生理学特性和机理提供了有价值的信息,鉴定获得的多聚半乳糖醛酸酶基因为深入研究揭示其在薇甘菊颈盲蝽与寄主互作中的作用奠定了基础。
Mikania micranthus Pachypeltis micranthus Mu et Liu (Hemiptera: Miridae) is a natural enemy of Mikania micrantha, a worldwide malignant weed. During the host's feeding of the natural enemies, polygalacturonase present in its saliva plays an important role as an important pectin hydrolase in degrading the host plant cell wall and causing tissue damage in the host plant. However, to date, no gene information on this natural enemy has been reported. The aim of this study was to obtain its transcriptome data and identify polygalacturonase genes based on this data. Transcriptome analysis using Illumina sequencing technology resulted in a total amount of data of approximately 5.2 Gbps, resulting in 57 739 unigene. By BlastX annotation, 23 549 non-redundant genes matched well-known proteins in the NCBI (Non-redundant Protein Database) NCBI, 7 048,8 548 and 16 135 non-redundant genes were successfully To GO (Gene Orthology), COG (Clustes of Orthologous Groups) and KEGG (Kyoto Encyclopedia of Genes and Genomes) databases. Based on the constructed transcriptome database, a total of 24 polygalacturonase genes were identified, of which 15 had a complete open reading frame. This transcriptome data will provide valuable information for further study on the ecological, biological and physiological characteristics and mechanism of Lygus lucorum at molecular level in the future. Identification of the obtained polygalacturonase gene for further study revealed It laid the foundation for the role of the lycaenidae and the host in Mikania micrantha.