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目的:探讨红景天苷和脑源性神经营养因子(BDNF)、神经干细胞(NSCs)共移植对致鼠NSCs定向分化影响。方法:将戊四氮致大鼠分为模型组、NSCs组、NSCs+BDNF组和NSCs+BDNF+红景天苷组。取新生大鼠海马组织,将培养的NSCs与BDNF+红景天苷+BDNF和基础培养基分别移植至致鼠海马组织中,苏木精-伊红染色及免疫组化检测不同时间点5-溴脱氧尿嘧啶核苷(BrdU)、谷氨酸脱羧酶(GAD65)阳性细胞数,并观察大鼠行为学改变。结果:NSCs+BDNF+红景天苷共移植组与其他组比较,各时间点BrdU、GAD65阳性细胞数均增多(P<0.05)。第3周开始,大鼠癫发作次数最少(P<0.05)。结论:BDNF与红景天苷联合有利于神经干细胞向γ-氨基丁酸能神经元分化。两者联合移植至致鼠后能减少大鼠的癫发作次数。
Objective: To investigate the effects of salidroside, brain-derived neurotrophic factor (BDNF) and neural stem cells (NSCs) co-transplantation on directional differentiation of murine NSCs. Methods: Rats in pentylenetetrazole group were divided into model group, NSCs group, NSCs + BDNF group and NSCs + BDNF + salidroside groups. The hippocampus of newborn rats were taken and the cultured NSCs and BDNF + salidroside + BDNF and basal medium were respectively transplanted into the hippocampus tissue of rats. The expression of 5-bromine Deoxyuridine (BrdU), glutamic acid decarboxylase (GAD65) positive cells, and observe the behavioral changes. Results: Compared with other groups, the number of BrdU and GAD65 positive cells in NSCs + BDNF + salidroside co-transplantation group increased at each time point (P <0.05). From the third week, the number of epileptic seizures in rats was the least (P <0.05). Conclusion: The combination of BDNF and salidroside is beneficial to the differentiation of neural stem cells into γ-aminobutyric acid-capable neurons. The combined transplantation of the two to the mouse reduced the number of epileptic seizures in rats.