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采用竞争性蛋白结合法测定了北京地区四个不同季节126例健康初产妇的母血及新生儿脐血的25-(OH)D_3.浓度其结果为母脐血的25-(OH)D_3经方差分析在季节间均有显著性差异,夏秋季高于冬春季.母血高于脐血,二者之间有明显正相关.经多元回归分析发现母脐血的25(OH)D_3与母孕后期户外活动时间、服VD(维生素D)的量、小儿身长呈正相关:而与小儿前匈大小、颅软程度、母亲孕后期腿抽搐程度呈负相关.我们还测定了12例母脐血的1.25-(OH)_2D_3,结果表明:母血的1.25-(OH)_2D_3明显高于脐血(P<0.001);母血的1.25-(OH)_2D_3无季节性差异并明显高于正常人的浓度.
The concentrations of 25- (OH) D 3 in maternal blood and newborn umbilical blood of 126 healthy primiparous women in four different seasons in Beijing were determined by competitive protein binding assay. The result was 25- (OH) D 3 ANOVA analysis showed significant differences between seasons, summer and autumn were higher than winter and spring, maternal blood was higher than umbilical cord blood, and there was a significant positive correlation between the two.According to multivariate regression analysis, 25 (OH) D_3 The amount of VD (vitamin D), the length of infants was positively correlated with the time of outdoor activities during the second trimester, but negatively correlated with the size of the anterior trimester of the fetus, the degree of cranial softness, and the degree of leg twitching in the second trimester of the mother.We also measured 12 maternal cord blood 1.25- (OH) _2D_3 in maternal blood was significantly higher than that in umbilical cord blood (P <0.001). The results showed that 1.25- (OH) _2D_3 in maternal blood was significantly higher than that in normal people concentration.