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目的:研究补肾健脾方治疗对急性肾病综合征(NS)模型大鼠病理结构的影响。方法:随机双盲法将32只Wistar雄性大鼠分为空白对照组、模型对照组、环磷酰胺组与联合组(环磷酰胺+补肾健脾方)各8只,共干预4周,比较4组干预前后体重、24小时尿蛋白定量、干预后大鼠血生化指标、性腺指标及病理结构特点。结果:环磷酰胺组、联合组分别与模型对照组比较在用药4周后体质量、24小时尿蛋白定量、TG、TP方面差异均有统计学意义(P<0.05)。联合组用药4周后24小时尿蛋白定量、TG、TP分别为(33.2±6.7)mg/24h、(1.24±0.12)mmol/L、(62.4±3.1)g/L,较环磷酰胺组的(59.4±10.2)mg/24h、(1.71±0.0)mmol/L、(55.7±6.8)g/L差异有统计学意义(P<0.05)。环磷酰胺组、联合组大鼠肾小球基本正常,肾间质变化不明显;环磷酰胺组睾丸生精细胞显著减少,精子数量小,而联合组生精细胞多且结构清除,精子细胞及精子数量比正常组织略低。结论:补肾健脾方能明显改善急性NS大鼠临床症状,且对其睾丸组织结构有一定的保护作用。
Objective: To study the effect of Bushen Jianpi Fang on the pathology of rats with acute nephritic syndrome (NS). Methods: 32 Wistar male rats were randomly divided into blank control group, model control group, cyclophosphamide group and combination group (cyclophosphamide + Bushen Jianpi Decoction) by random double-blind method for a total of 4 weeks. Four groups before and after intervention, body weight, 24-hour urinary protein, blood biochemical indicators after intervention, gonadal indicators and pathological features. Results: Compared with the model control group, the differences of body weight, 24 hour urinary protein, TG and TP in the cyclophosphamide group and the combination group were statistically significant (P <0.05). The urinary protein in 24 hours after the combination therapy in the combined group was (33.2 ± 6.7) mg / 24h, (1.24 ± 0.12) mmol / L and (62.4 ± 3.1) g / L respectively (59.4 ± 10.2) mg / 24h, (1.71 ± 0.0) mmol / L and (55.7 ± 6.8) g / L, respectively (P <0.05). Cyclophosphamide group, combined group of rats glomerular normal, renal interstitial changes was not obvious; Cyclophosphamide group testicular spermatogenic cells was significantly reduced, sperm count is small, and combined group of spermatogenic cells and more clear structure, sperm cells And sperm count slightly lower than normal tissue. Conclusion: Bushenjianpi Recipe can significantly improve the clinical symptoms of acute NS rats and protect the structure of testis tissue.