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目的:研究三物白散对胃癌耐药细胞株SGC7901/DDP多药耐药(MDR)的逆转作用,明确其耐药逆转机制。方法:分别以不同浓度的三物白散作用于体外培养的SGC7901和SGC7901/DDP细胞,以MTT法检测细胞增殖抑制率,确定三物白散的非毒性剂量;以不同浓度顺铂(DDP)作用上述细胞,确定没有三物白散干预时各组细胞生长抑制50%的DDP浓度(IC50)和SGC7901/DDP细胞的耐药指数(RF);采用非毒性剂量三物白散,分别与不同浓度顺铂(DDP)联合作用,检测各组细胞生长抑制50%的DDP浓度,计算RF和耐药逆转倍数(RI);利用流式细胞术检测三物白散联合DDP作用后SGC7901/DDP细胞内DDP的荧光强度、P-gp的表达和P-gp功能活性。结果:三物白散的非细胞毒性剂量为10-7g/mL;单独顺铂作用后SGC7901/DDP细胞的耐药指数(RF)为3.94;三物白散协同DDP作用后,SGC7901/DDP细胞的RF为3.75,RI为6.12;与三物白散未干预组比较,三物白散可显著增加DDP在SGC7901/DDP细胞内的蓄积(P<0.05),SGC7901/DDP细胞的P-gp表达亦显著降低(P<0.01),而P-gp功能显著抑制(P<0.01)。结论:三物白散与DDP联合运用既有复合抗癌之效,又能逆转胃癌细胞SGC7901/DDP多药耐药。其主要机制可能是通过三物白散下调SGC7901/DDP细胞P-gp表达、抑制P-gp功能实现的。
Objective: To investigate the reversal effect of San Wu Bai San on multidrug resistance (MDR) in gastric cancer cell line SGC7901 / DDP and to clarify the reversal mechanism of drug resistance. METHODS: SGC7901 and SGC7901 / DDP cells were cultured with different concentrations of Sanwualitang. The inhibitory rates of cell proliferation were determined by MTT assay, and the non-toxic dose of Sanwuibaisan was determined. With different concentrations of cisplatin (DDP) The above-mentioned cells were used to determine the IC50 of 50% inhibition and the drug resistance index (SGR) of SGC7901 / DDP cells in each group without intervention of Sanwuosan; (DDP), the concentration of DDP in 50% inhibition of cell growth in each group was detected, and the RF and reversal of drug resistance (RI) were calculated. The flow cytometry was used to detect SGC7901 / DDP cells Fluorescence intensity, P-gp expression, and P-gp functional activity within DDP. Results: The non-cytotoxic dose of Sanwuibaisan was 10-7g / mL; the drug resistance index (SGR) of SGC7901 / DDP cells was 3.94 after treated with cisplatin alone; SGC7901 / DDP cells (P <0.05). The P-gp expression in SGC7901 / DDP cells was significantly higher than that in the control group (P <0.01), while P-gp function was significantly inhibited (P <0.01). Conclusion: The combination of Sanwuibalian and DDP not only has the effect of compound anticancer but also reverses the multidrug resistance of SGC7901 / DDP in gastric cancer cells. Its main mechanism may be through the three things Baichang SGC7901 / DDP down-regulation of P-gp expression, inhibition of P-gp function achieved.