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目的 分析重组HIV-1-p24抗原,以便研制HIV-抗体诊断试剂。方法 以纯化重组P24为包被抗原,应用ELISA检测HIV-Ab国家参比品和正常人血清。结果20份阳性HIV-Ab检出阳性19份,符合率为95％;20份阴性HIV-Ab检出阴性18份,符合率为90％;50份正常人血清检测结果均为阴性。结论 所研制的重组P24抗原具有较高的特异性和敏感性,可用于制备HIV抗体诊断试剂。
Objective To analyze recombinant HIV-1-p24 antigen in order to develop HIV-antibody diagnostic reagents. Methods Purified recombinant P24 was used as coating antigen, and HIV-Ab national reference material and normal human serum were detected by ELISA. RESULTS: Twenty-nine positive HIV-Abs were detected positive, with the coincidence rate of 95%; 20 negative HIV-Ab negative samples were detected negative, with a coincidence rate of 90%; 50 normal human serum test results were negative. Conclusion The recombinant P24 antigen has high specificity and sensitivity and can be used to prepare HIV antibody diagnostic reagent.