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番茄的多聚半乳糖醛酸是一种在果实成熟阶段特异性表达的酶。为了研究它在果实成熟中的作用,将其cDNA与花椰菜花叶病毒355启动子嵌合后,以反义基因的形式经农杆菌介导导入番茄植株,进一步分析了反义基因的整合与表达。结果表明,在转基因番茄中,反义基因的表达能明显抑制果实内源多聚半乳糖醛酸酶的活性。
Polygalacturonan from tomato is an enzyme that is specifically expressed during the fruit ripening stage. In order to study its role in fruit maturation, its cDNA was cloned into cauliflower mosaic virus 355 promoter and introduced into tomato plants via Agrobacterium tumefaciens antisense gene. The integration and expression of antisense gene were further analyzed . The results showed that in transgenic tomato, the expression of antisense gene could significantly inhibit the activity of endogenous polygalacturonase in fruits.