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利用制备性SDS—聚丙烯酰胺凝胶电泳(PAGE)进行人重组白细胞介素—3(IL—3)的纯化,回收率可达84.3%,纯度90%以上,保持高度生物活性,并有方法简便,实验周期短的优点,已小批量供应国内实验室进行人IL—3的体外研究.
Human recombinant interleukin-3 (IL-3) was purified by preparative SDS-polyacrylamide gel electrophoresis (PAGE) with a recovery of 84.3% and a purity of over 90%, maintaining a high degree of biological activity Simple, short cycle of the advantages of small quantities have been supplied to domestic laboratories for human IL-3 in vitro studies.