【摘 要】
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Objective: To observe the effect of MTA1 gene silencing by RNAinterference on invasion and migration of esoph-ageal carcinoma 9706 cells. Methods: siRNA express
【机 构】
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Department of Microbiology and Immunology, College of Basic Medical Science, Zhengzhou University,Zh
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Objective: To observe the effect of MTA1 gene silencing by RNAinterference on invasion and migration of esoph-ageal carcinoma 9706 cells. Methods: siRNA expression vector targeting MTA1 gene was transfected into EC9706 cells by Lipofectamine method. MTA1 mRNA and protein expressions were detected through quantitative RT-PCR and Western Blot, respectiuvely. The invasion and migration of EC9706 cells were evaluated by scrape wound healing assay and cell invasion as-say in vitro. Results: MTA1 gene expression decreased significantly. The scrape wound of EC9706 cells healed more slowly and the cell popuiaUon that cut through Matrigel were less in the EC9706 ceils transfected with siRNA expression vector than non-transfected EC9706 cells and EC9706 cells transfected with blank vector (P < 0.05). Conclusion: MTA1 gene silencing by RNAi can inhibit the invasion and migration of esophageal carcinoma effectively. It is supposed that MTA1 gene may be a prospective molecule target in tumor therapy.rn
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