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利用苹果基因组筛选K~+转运蛋白基因,分析其系统发育关系,通过q RT-PCR检测它们在平邑甜茶各器官组织和不同发育阶段果实的表达特征。结果表明,在苹果中存在65个K~+转运蛋白基因,包括CHX家族(33个)、HAK家族(24个)、HKT家族(1个)和KEA家族(7个),它们与拟南芥K~+转运蛋白基因高度同源,其基因结构相对保守,并且不均匀地分布在13条染色体上。定量表达分析发现,K~+转运蛋白基因具有不同的表达模式,其中在根中高度表达的32个,在叶片中高度表达的12个,在茎尖中高度表达的11个,在果实中高度表达的19个。研究结果为揭示苹果K~+转运蛋白基因的功能提供了基础资料。
The K + transporter genes were screened from the apple genome and their phylogenetic relationships were analyzed. The expression patterns of these genes were detected by q RT-PCR in various organs and tissues at different developmental stages. The results showed that there are 65 K ~ + transporter genes in apple, including CHX family (33), HAK family (24), HKT family (1) and KEA family (7) K ~ + transporter gene highly homologous, its gene structure is relatively conservative, and unevenly distributed in 13 chromosomes. Quantitative expression analysis revealed that the K ~ + transporter genes have different expression patterns, of which 32 were highly expressed in roots, 12 highly expressed in leaves, 11 highly expressed in shoot tips, and highly expressed in fruits 19 expressed. The results provide basic information for revealing the function of K ~ + transporter gene in apple.