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建立一种高效液相色谱法,对43.5%吡虫·氟虫腈悬浮种衣剂中有效成分进行分离和定量分析。方法使用HYPERSIL BDS-C18色谱柱和紫外可变波长检测器,以甲醇+水为流动相,在268nm波长下采用外标法定量。氟虫腈的标准偏差为0.045 3,变异系数为0.32%,回收率为98.3%~100.3%;吡虫啉的标准偏差为0.107 9,变异系数为0.37%,回收率为99.6%~100.2%。
A high performance liquid chromatography (HPLC) was developed to separate and quantify the active compounds in the suspension of 43.5% imidacloprid and fipronil. Methods Using HYPERSIL BDS-C18 column and UV-vis detector with methanol + water as mobile phase, the method was quantified by external standard method under the wavelength of 268 nm. The standard deviation of fipronil was 0.045 3, the coefficient of variation was 0.32% and the recovery rate was 98.3% -100.3%. The standard deviation of imidacloprid was 0.107 9, the coefficient of variation was 0.37% and the recovery rate was 99.6% -100.2%.