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黑木耳耳片组织分离菌种,一般先用水将耳片泡开,再行药液表面灭菌,尔后取处理好的组织块置斜面上培养。这种方法在应用时不易掌握,不是药液将组织块杀死,就是组织块吸水过多不萌发或污染。为克服此缺点,我们采用干耳分离法。将晒至足干的耳片连同试管,在分离前同时放入接种箱,用常规剂量的甲醛薰蒸40分钟,进行无菌操作,取
Black fungus ear tissue isolation strains, the general first ear bubble with water, and then the surface of the liquid sterilized, and then take a good deal of tissue placed slant on the slant. This method is not easy to grasp in the application, not the liquid will kill the tissue block, tissue block is too much water does not germinate or contaminated. To overcome this shortcoming, we use the dry ear separation method. The sun dry enough to dry ears together with the test tube, into the inoculation box at the same time before separation, with conventional dosage of formaldehyde fumigation 40 minutes, aseptic operation, take