Co-expression of Clostridium perfringens α and ε Toxins and Their Immunogenicity

• 作 者: Han Guangwei Li Zhaocai Gong X
• 机 构: StateKeyLaboratoryofVeterinaryEtiologicalBiology/LanzhouVeterinaryResearchInstitute,LanzhouAnimalHea
• 出 处: 动物与饲料科学：英文版
• 发表日期: 2015年2期
• 关键词: 产气荚膜梭菌 共表达质粒 毒素蛋白 免疫原性 SDS-PAGE分析 免疫小鼠 培养滤液 PCR扩增 Clostridium perfingens α toxi
• 基金项目: Supported by the Basic Scientific Research Project of Nonprofit Central Research Institutions （1610322013028）.

The gene CPA and ETX encoding α and ε toxins were amplified from the standard strain C60-2 of type D Clostridium perfingens by PCR,and then they were inserted into pet-Duet-1 vecto,respectively for the construction of co-expression plasmid p ETDuet-1-CPA-