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本文于硅胶 G 薄层上用异丙醚∶甲醇∶正丁醇(3∶0.5∶0.5)分离了土大黄甙;用正丙醇∶乙酸乙酯∶水(4∶4∶3)分离了番泻甙 A 及 B;用石油醚∶己烷∶甲酸乙醣∶甲酸(1∶3∶1.5∶0.1)加蒸溜水0.5ml 振摇分取上层液及苯∶甲酸乙酯∶甲酸∶甲醇(3∶1∶0.05∶0.2)加蒸溜水0.5ml 振摇分取上层液两个溶剂系统为展开剂分离了大黄中的大黄酚、大黄素甲醚、大黄素、芦荟大黄素及大黄酸等五种蒽醌甙元,并研究成功了生药提取的改良方法,以及应用岛津 CS-900双波长薄层扫描仪以反射法直线扫描于样品波长(λs)420nm,参考波长(λR)650nm 处,分别测定了上述五种蒽醌甙元的含量,方法快速、简便、灵敏、稳定。实验结果证明:1.中国大黄中总蒽醌含量幅度为1～8%,不同
In this paper, terbinaquinone was isolated on a thin layer of silica gel G with isopropyl ether:methanol:n-butanol (3:0.5:0.5); the separation was carried out with n-propanol:ethyl acetate:water (4:4:3). Cirrhea A and B; add 0.5 ml of distilled water with petroleum ether:hexane:formic acid ethyl sugar:formic acid (1:3:1.5:0.1), shake and collect the supernatant and benzene: ethyl formate: formic acid: methanol (3 1:1:0.05:0.2) Add distilled water 0.5ml Shake and take two liquids of the upper layer solution as the developing agent Five kinds of rhubarb, emodin, emodin, aloe-emodin and rhein in rhubarb were isolated.蒽醌甙元, and has studied successfully the improved method of crude drug extraction, and the application of Shimadzu CS-900 dual-wavelength thin-layer scanner scanning linearly at the sample wavelength (λs) 420nm, reference wavelength (λR) 650nm, respectively, The contents of the above five kinds of aglycons were determined. The method is fast, simple, sensitive, and stable. The experimental results show that: 1. The content of total germanium in Chinese rhubarb is 1-8%, different