论文部分内容阅读
在未加和加入体外代谢激活体系S9混合物2种情况下,将人精子用最终浓度分别为20、40、60μg/ml环磷酰胺处理3.5h,然后与去透明带地鼠卵进行异种体外受精,继而制备精子染色体进行核型分析。在未加S9混合物组,环磷酰胺3种剂量处理的染色体结构畸变精子率依次为10％、12％、10％;断裂均数依次为0.22、0.14、0.20;在加入S9混合物组,畸变精子率依次为18％、24％、32％;断裂均数依次为0.38、0.78、1.64.2组间畸变精子率和断裂均数差异显著(P<0.05)。加入S9混合物组,环磷酰胺3种剂量处理的畸变精子率和断裂均数都高于空白对照组(10％,0.12％),其差异具有统计学意义且畸变精子率和断裂均数随着环磷酰胺剂量增加而增高。本研究结果表明,人精子染色体离体测试系统可以用来检测原诱变剂对人精子中遗传物质的诱变效应。但原诱变剂必须经S9混合物代谢成激活型。
Without adding S9 mixture in vitro, the human sperm were treated with cyclophosphamide at the final concentration of 20, 40 and 60μg / ml for 3.5h respectively, and then inoculated with zona pellucida ova for in vitro in vitro fertilization , Followed by preparation of sperm chromosome for karyotyping. In the group without S9 mixture, the structural aberration rate of sperm in the three doses of cyclophosphamide were 10%, 12% and 10%, respectively; the average number of breakage was 0.22, 0.14 and 0.20 respectively. After adding S9 mixture, The rates of rupture were 18%, 24% and 32%, respectively. The mean number of rupture was 0.38,0.78 and 1.64 respectively. There was significant difference between the two groups (P <0.05). The S9 mixed group, cyclophosphamide three kinds of doses of the treatment of abnormal sperm rate and fracture mean were higher than the blank control group (10%, 0.12%), the difference was statistically significant and the average number of abnormal sperm and fracture along with Cyclophosphamide dose increased and increased. The results of this study show that human sperm chromosome in vitro test system can be used to detect the mutagenic effect of mutagens on human sperm genetic material. However, the original mutagen must be metabolized by the S9 mixture to an active form.