Separation and Purification of Total Phloroglucinols inDryopteris crassirhizoma with DM-130 Macropor

• 作 者: Jin Zhe Huang Jian-ping Wang He-meng Ju han-xun Ren Si-rui Chang Ying
• 机 构: College of Life Sciences, Northeast Agricultural University, Harbin 150030, China
• 出 处: 东北农业大学学报（英文版）
• 发表日期: 2015年2期
• 关键词: DM-130 adsorption resin Dryopteris crassirhizoma total phloroglucinols separatio

To improve the purity of the total phloroglucinols fromDryopteris crassirhizoma extracts, the separation and purification conditions of the total phloroglucinols fromDryopteris crassirhizoma were studied with DM-130 macroporous adsorption resin in this study. Adsorption rate, elution rate and purity of the total phloroglucinols were used as indexes to investigate the adsorption and desorption capacity of the total phloroglucinols with DM-130 macroporous adsorption resin. Through the study, the optimum sample concentration of the total phloroglucinols and maximum sample volume were 1.5 mg? mL-1 and 7 BV (210 mL), respectively. The optimum desorption conditions were achieved by using 80% ethanol as desorption solvent at elution flow rate of 1.0 mL? min-1. The result showed DM-130 macroporous adsorption resin performed effective adsorption and desorption. After purification, the purity of the total phloroglucinols increased by 11.5-fold.