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姊妹染色单体分化(简称SCD)染色法是七十年代发展起来的新技术。是检测诱变剂或致癌剂的一种高度灵敏的方法。鉴于目前已知的一些诱变剂或致癌剂即使其浓度在细胞毒理学和致染色体断裂水平以下时,也能诱发姊妹染色体互换(简称SCE)且发现化学物质致突变的能力与它导形成SCE的能力之间存在着相关性。因而,测定化学物质或天然产物的诱发SCE的能力,可用于检定染色体畸变的良好指标。本文用SCD技术测定了两种植物药:从竹节香附根茎中
Sister chromatid differentiation (SCD) staining is a new technique developed in the 1970s. It is a highly sensitive method for detecting mutagens or carcinogens. Given that some currently known mutagens or carcinogens, even at concentrations below the level of cytotoxicity and chromosomal breaks, can induce sister chromatid exchanges (SCEs) and have the ability to detect and induce chemical mutagenesis. There is a correlation between the capabilities of SCEs. Thus, determining the ability of a chemical or natural product to induce SCE can be used to test for good indicators of chromosomal aberrations. In this paper, two kinds of botanical drugs were determined by SCD technology: