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目的:采用高效液相色谱法同时测定9批不同产地灯盏细辛药材中绿原酸、咖啡酸、1,5-二-O-咖啡酰奎宁酸和野黄芩苷4种有效成分。方法:采用ZorbaxC18色谱柱(4.6mm×150mm,5μm),以乙腈(A)-0.05%磷酸水溶液(B)为流动相梯度洗脱(0～9min,10%A;9～10min,10%A～13%A;10～40min,13%A),流速1.0mL·min-1,柱温30℃,检测波长325nm。结果:绿原酸、咖啡酸、1,5-二-O-咖啡酰奎宁酸、野黄芩苷浓度分别在3.93～62.9μg·mL-1(r=0.9999)、0.106～1.70μg·mL-1(r=0.9997)、0.124～1.98μg·mL-1(r=0.9995)、28.78～460.4μg·mL-1(r=0.9999)范围内呈现良好的线性关系,平均回收率(n=6)分别为95.74%(RSD=1.7%)、103.3%(RSD=2.6%)、100.0%(RSD=4.0%)、101.8%(RSD=3.4%)。结论:该方法简便、准确,可用于灯盏细辛药材的质量评价。
OBJECTIVE: To simultaneously determine four active ingredients of chlorogenic acid, caffeic acid, 1,5-di-O-caffeoylquinic acid and wild baicalin in 9 batches of Erigeron breviscapus from different areas by high performance liquid chromatography. METHODS: The mobile phase was eluted with a mobile phase of acetonitrile (A) - 0.05% phosphoric acid (B) (0-9 min, 10% A; 9-10 min, 10% A) using a Zorbax C18 column (4.6 mm × 150 mm, ~ 13% A; 10 ~ 40min, 13% A), the flow rate was 1.0mL · min-1, the column temperature was 30 ℃ and the detection wavelength was 325nm. Results: The concentrations of chlorogenic acid, caffeic acid, 1,5-di-O-caffeoylquinic acid and baicalin were 3.93-62.9 μg · mL -1 (r = 0.9999) and 0.106-1.70 μg · mL -1 (R = 0.9997), 0.124-1.98μg · mL-1 (r = 0.9995), 28.78-460.4μg · mL-1 (r = 0.9999). The average recovery (n = 6) They were 95.74% (RSD = 1.7%), 103.3% (RSD = 2.6%), 100.0% (RSD = 4.0%) and 101.8% (RSD = 3.4%) respectively. Conclusion: The method is simple and accurate and can be used for quality evaluation of Erigeron Breviscapus.