聚合酶链反应检测痰样品中军团病杆菌核酸

• 作 者: 张立平 王祥 殷凯生 俞婉珍 李淑云 付完珍
• 机 构: 溧水县人民医院检验科,南京医科大学一附院呼吸科
• 出 处: 临床检验杂志
• 发表日期: 1998年02期
• 关键词: 军团病杆菌 军团菌病 聚合酶链反应 诊断阳性率 急性期 巨噬细胞 肺部感染患者 血清学阳性 增效基因 临床诊断

用聚合酶链反应（ＰＣＲ）对４０例有军团病杆菌（ＬＤＢ）培养和／或血清学阳性证据，临床诊断为军团菌肺炎患者急性期的痰样品中军团菌巨噬细胞感染增效基因（ｍｉｐｇｅｎｅ）的６３０ｂｐ片段进行ＤＮＡ扩增，诊断阳性率为８２．５％（３３／４０）。同时用ＰＣＲ检测了收集的其中２９位患者的急性期支气管肺泡灌洗液（ＢＡＬＦ）样品，ＢＡＬＦ诊断阳性为８９．７％（２６／２９），急性期痰样品与ＢＡＬＦ样品中ＬＤＢ的ＰＣＲ检出率之间无显著性差异（χ２＝０．６９，Ｐ＞０．２５）。

Polymerase chain reaction (PCR) was performed on 40 cases of Legionella pneumophila infection and / or serological positive evidence of clinical diagnosis of Legionella pneumophila sputum samples in patients with Legionella pneumophila infection of the synergistic effect of the gene ( mipgene 630bp fragment of DNA amplification, the diagnostic positive rate was 82.5% (33/40). A total of 29 patients with acute bronchial alveolar lavage fluid (BALF) samples were collected and analyzed by PCR. The positive results of BALF were 89.7% (26/29). The PCR results of acute sputum and LDB in BALF There was no significant difference between rates (χ2 = 0.69, P> 0.25).