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目的研究姜黄素对人肾癌786-О细胞体外生长及细胞周期的影响,为肾癌治疗提供理论依据。方法应用MTT比色法和流式细胞仪检测人肾癌786-О细胞增殖抑制率及细胞周期各时相的变化和凋亡率。结果姜黄素对人肾癌786-О细胞有抑制作用,存在剂量依赖(F=6207.813,P<0.01)与时间依赖(F=1210.386,P<0.01);流式细胞仪分析,G1期细胞增多,S期细胞减少,C2/M期细胞相对增多。结论姜黄素可以抑制人肾癌786-О细胞增殖,阻止G1期细胞向S期的进程,促进人肾癌786-О细胞凋亡。
Objective To study the effects of curcumin on the growth and cell cycle of human renal cell carcinoma 786-o so as to provide a theoretical basis for the treatment of renal cell carcinoma. Methods MTT colorimetric assay and flow cytometry were used to detect the cell proliferation inhibitory rate, cell cycle phase change and apoptosis rate of human renal carcinoma 786-O cells. Results Curcumin inhibited human renal carcinoma 786-O cells in a time-dependent manner (F = 6207.813, P <0.01) and time-dependent manner (F = 1210.386, , S phase cells decreased, C2 / M phase cells increased relative. Conclusion Curcumin can inhibit the proliferation of human renal carcinoma 786-O cells and prevent the progression of G1 phase to S phase and promote the apoptosis of human renal carcinoma 786-O cells.