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类风湿性关节炎(rheumatoid arthritis,RA)是常见的以关节滑膜慢性炎症为主要特征的自身免疫性疾病,其主要病理改变为关节滑膜明显增厚并有大量炎症细胞浸润,血管翳形成以及软骨组织的侵蚀与破坏[1]。microRNA可被分泌释放到细胞外,参与多种炎性和自身免疫性疾病的发病过程[2]。Micro-RNAs(miRNAs)是一类短的(大约20～22个核苷酸)非编码RNA,介导RNA干扰和在翻译水平抑制蛋白表达。miRNAs与自身免疫性疾病进程有关且可能参与RA新的调控机制[2-4]。miRNA-146a与类风湿性关节炎密切相关,TNF-α[5]和IL-17[6]目前被认为是RA的一种重要致炎因子,均与疾病活动程度相关;而C-反应蛋白(C-reactive protein,CRP)[7]是一种急性时相反应蛋白,可以反映患者的疾病活性;一直以来,类风湿因子(rheumatoid factor,RF)是美国风湿病协会RA诊断标准中的血清学指标之一[8-9];因此本研究采用实时荧光定量PCR检测miR-146a在RA患者外周血单个核细胞(peripheral blood mononu-clear cell,PBMC)表达水平,同时检测血清CRP、RF、IL-I7、TNF-α含量,分析miR-146a在RA外周血中的表达及与CRP、RF、IL-I7、TNF-α的相关性,有助于进一步探讨其在RA发病及疾病进展中的意义。
Rheumatoid arthritis (RA) is a common autoimmune disease characterized by chronic synovial inflammation. Its main pathological changes are thickening of synovium and infiltration of a large number of inflammatory cells and angiogenesis As well as the erosion and destruction of cartilage tissue [1]. MicroRNAs can be secreted and released extracellularly, and are involved in the pathogenesis of many inflammatory and autoimmune diseases [2]. Micro-RNAs (miRNAs) are a class of short (about 20 to 22 nucleotides) non-coding RNA that mediates RNA interference and inhibits protein expression at the translational level. MiRNAs are involved in the process of autoimmune diseases and may be involved in the new regulatory mechanisms of RA [2-4]. MiRNA-146a is closely related to rheumatoid arthritis. TNF-α [5] and IL-17 [6] are currently considered as important inflammatory factors of RA and are related to disease activity. C-reactive protein (C-reactive protein, CRP) [7] is an acute phase reaction protein that can reflect the patient’s disease activity. The rheumatoid factor (RF) has long been the serum of the American College of Rheumatology RA diagnostic criteria Therefore, in this study, real-time fluorescent quantitative PCR was used to detect the expression of miR-146a in peripheral blood mononuclear cells (PBMCs) of patients with RA. Serum CRP, RF, IL-I7 and TNF-α, and to analyze the correlation between the expression of miR-146a in RA peripheral blood and CRP, RF, IL-I7 and TNF-α, so as to further explore its role in the pathogenesis and progression of RA Meaning.