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背景:移植后造血干细胞的顺利归巢是单倍相合骨髓移植成功的关键。目的:构建小鼠白血病模型,进行单倍型相合的骨髓移植,动态观察受体小鼠体内移植细胞归巢率及不同时间点的归巢规律。设计、时间及地点:随机对照观察实验,于2005-11/2007-10在福建医科大学实验动物中心清洁级动物实验室进行。材料:供鼠为清洁级近交系C57BL/6小鼠和C57BL/6小鼠与BALB/c小鼠杂交的雄性F1小鼠,受鼠为清洁级近交系雌性BALB/c小鼠,用于构建白血病小鼠模型。方法:将48只造模小鼠随机分为2组,对照组供鼠为C57BL/6小鼠,实验组供鼠为F1小鼠。实验组进行主要组织相容性复合物单倍型相合的同种异基因骨髓移植,对照组进行主要组织相容性复合物完全不相合的骨髓移植。主要观察指标:移植后1,2,3,4d动态观察小鼠外周血、骨髓和脾中有核细胞计数,以流式细胞仪检测可与FITC标记的抗鼠H-2Kb单抗结合的供鼠阳性细胞并计算骨髓和脾归巢率。结果:对照组外周血中供鼠来源的细胞数持续下降,脾和骨髓的归巢率均为先升后降,提示供者来源的骨髓细胞在受鼠体内为先归巢再出巢;实验组外周血中供鼠来源的细胞数先下降,再持续上升,骨髓归巢率为下降后上升,脾归巢率为上升后下降,提示供鼠来源骨髓细胞在骨髓中表现为出巢后再归巢,脾中有延迟,与对照组比较,实验组第4天外周血中供鼠细胞数量及骨髓和脾归巢率均增高(P<0.05)。结论:主要组织相容性复合物单倍体相合的骨髓移植的归巢规律是先出巢再归巢,归巢的细胞数量和归巢率高于主要组织相容性复合物完全不相合骨髓移植。
Background: The successful homing of hematopoietic stem cells after transplantation is the key to successful haploidentical bone marrow transplantation. OBJECTIVE: To construct a murine leukemia model and perform haploidentical bone marrow transplantation to observe dynamically the homing rate of transplanted cells in vivo and the homing patterns at different time points. DESIGN, TIME AND SETTING: A randomized controlled observational study was performed at the Laboratory of Animal Purification Laboratory, Fujian Medical University from November 2005 to October 2007. MATERIALS: Male F1 mice were crossed with BALB / c mice in clean-grade inbred line C57BL / 6 mice and C57BL / 6 mice. The mice were clean grade inbred female BALB / c mice. To construct leukemia mouse model. Methods: Forty-eight mice were randomly divided into two groups. The control group was given C57BL / 6 mice and the experimental group was F1 mice. The experimental group was performed haploidentical allogeneic bone marrow transplantation of the major histocompatibility complex, and the control group was completely mismatched with the major histocompatibility complex bone marrow transplantation. MAIN OUTCOME MEASURES: The number of nucleated cells in peripheral blood, bone marrow and spleen of mice was dynamically observed on the 1st, 2nd, 4th and 4th days after transplantation. The binding of FITC-labeled anti-mouse H-2Kb mAb Murine positive cells were counted and bone marrow and spleen homing rates were calculated. Results: In the control group, the number of donor cells in the peripheral blood continuously decreased, and the homing rates of spleen and bone marrow increased first and then decreased, suggesting that the donor bone marrow cells homing first and then out of the nest. The number of cells in the peripheral blood of the rats for the first time decreased, and then continued to rise, the rate of bone marrow homing decreased and then increased, and the rate of spleen homing increased and then decreased, suggesting that the bone marrow cells from the donor mice appeared in the bone marrow Homing, spleen delay, compared with the control group, experimental group on the 4th day of peripheral blood donor rat cells and bone marrow and spleen homing rate was increased (P <0.05). CONCLUSIONS: Homologous rule of haploidentical bone marrow transplantation of major histocompatibility complex is that the homing rule is that the number of homing cells and homing rate is higher than that of the major histocompatibility complex completely disagreeing with bone marrow transplant.