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目的:建立党参与秦艽的非线性电化学鉴别方法。方法:在玻璃反应器中加入粒径小于0.125 mm的中草药粉一定量(准确到0.002 g)作为反应的底物,采用H+-Mn2+-CH3COCH3-Br O-3为振荡体系,控制温度310 K和搅拌速度500 r·min-1。以饱和甘汞电极作参比,铂电极作指示电极,采用电化学工作站记录化学振荡体系中电位E随时间t的变化,利用Origin 7.5软件绘制E-t曲线,获得非线性电化学指纹图谱。考察图谱的特征参数:最高电位、诱导时间、振荡寿命、振荡周期,计算反应的活化能,运用聚类分析法对其进行研究。结果:非线性电化学指纹图谱的重复性良好,直观上看党参与秦艽的非线性电化学指纹图谱具有明显的差异,特征参数也有明显的不同,聚类分析将样品分为两类。结论:可以利用此方法鉴别党参与秦艽。
Objective: To establish a non-linear electrochemical identification method for the party to participate in Gentiana macrophylla. Methods: A certain amount (accurate to 0.002 g) of Chinese herbal powder with a particle size of less than 0.125 mm was added into the glass reactor as the reaction substrate. H + -Mn2 + -CH3COCH3-Br O-3 was used as the oscillating system to control the temperature of 310 K and Stirring speed 500 r · min-1. Using saturated calomel electrode as reference electrode and platinum electrode as indicator electrode, electrochemical workstation was used to record the change of potential E in the chemical oscillation system with time t. Origin 7.5 software was used to draw E-t curve to obtain nonlinear electrochemical fingerprint. The characteristic parameters of the spectrum were investigated: the highest potential, the induction time, the oscillation life and the oscillation period, and the activation energy of the reaction was calculated. The cluster analysis was used to study the characteristic parameters. Results: The repeatability of non-linear electrochemical fingerprints was good. The nonlinear electrochemical fingerprints of Codonopsis pilosula and Gentiana macrophylla showed obvious differences, and the characteristic parameters were obviously different. Cluster analysis divided the samples into two categories. Conclusion: This method can be used to identify the participation of the party in the Gentiana.