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目的:研究参附注射液对大鼠心肌肥大模型microRNA-let-7e-5p的调控机制。方法:取健康SD大鼠行腹主动脉缩窄手术建立心肌肥大模型,分成3组:参附注射液组(SFI,6.0m L·kg-1·d-1);模型组(0.9%氯化钠溶液,6.0m L·kg-1·d-1);假手术组(0.9%氯化钠溶液,6.0m L·kg-1·d-1);腹腔注射相应药物,连续12周。采用茎环Realtime RT-PCR方法检测大鼠心肌microRNA-let-7e-5p的表达。利用生物信息学方法分析microRNA-let-7e-5p的候选靶基因,并用RT-PCR与Western Blot方法检测靶基因Caspase-3的m RNA与蛋白表达水平。结果:模型组大鼠心肌mi RNA-let-7e-5p下调,Caspase-3 m RNA及蛋白过表达;参附注射液组大鼠心肌microRNA-let-7e-5p表达量、Caspase-3 m RNA及蛋白表达水平与假手术组接近。结论:mi RNA-let-7e-5p在心肌肥大中可能发挥重要作用,参附注射液可能通过上调或维持mi RNA-let-7e-5p的表达,降低Caspase-3的水平,抑制腹主动脉缩窄大鼠心室重构,从而改善大鼠心功能,对心肌肥大有明显的治疗作用。
Objective: To study the regulatory mechanism of Shenfu injection on rat cardiac hypertrophy model microRNA-let-7e-5p. Methods: The model of cardiac hypertrophy was established by abdominal aorta constriction in healthy SD rats. The rats were divided into 3 groups: SFI (6.0m L · kg-1 · d-1); model group (0.9% Sodium hydride solution, 6.0m L · kg-1 · d-1). Sham operation group (0.9% sodium chloride solution, 6.0m L · kg-1 · d-1) was injected intraperitoneally for 12 weeks. Realtime RT-PCR was used to detect the expression of microRNA-let-7e-5p in rat myocardium. Candidate target genes of microRNA-let-7e-5p were analyzed by bioinformatics methods. The mRNA and protein levels of target gene Caspase-3 were detected by RT-PCR and Western Blot. Results: The expression of miRNA-let-7e-5p and Caspase-3 mRNA and protein in myocardium of model group were down-regulated. The expression of microRNA-let-7e-5p, And protein expression level close to the sham group. Conclusion: Mi RNA-let-7e-5p may play an important role in myocardial hypertrophy. Shenfu injection may inhibit the expression of miRNA-let-7e-5p, decrease the level of Caspase-3, Narrowing rat ventricular remodeling, thereby improving cardiac function in rats, myocardial hypertrophy obvious therapeutic effect.