论文部分内容阅读
本实验的目的是评价血小板不同密度亚群对不同的血小板聚集剂的反应。研究结果表明，以间断梯度离心法，可将家兔血小板分为高密度（ＨＤＰ）、中密度（ＩＤＰ）和低密度（ＬＤＰ）三个亚群。ＨＤＰ亚群对胶原（ＣＯｌｌｇｅｎ）、花生四烯酸（ＡＡ）、血栓素（ＴＸＡ２）同系物（ＳＴＡ２）、腺苷二磷酸（ＡＤＰ）及Ｃａｌｃｉｕｍｉｏｎｏｐｈｏｒｅ（Ａ２３１７８）的血小板聚集和ＡＴＰ释放反应，都明显强于其它两个亚群（Ｐ＜０．０５或Ｐ＜０．０１）。单一血小板细胞内游离钙的测定结果也证实ＨＤＰ亚群的细胞内钙动员显著高于ＩＤＰ和ＬＤＰ两亚群。结论，ＨＤＰ亚群血小板的功能反应性强，对聚集剂最敏感。
The purpose of this experiment was to evaluate the response of different density platelets to different platelet aggregates. The results show that the rabbit platelets can be divided into three subgroups: high density (HDP), medium density (IDP) and low density (LDP) by means of discontinuous gradient centrifugation. The platelet aggregation and ATP release responses of HDP subpopulations to COllgen, arachidonic acid (AA), TXA2 homologues (STA2), adenosine diphosphate (ADP) and Calcium ionophore (A23178) Stronger than the other two subgroups (P <0.05 or P <0.01). The determination of intracellular free calcium in a single platelet also confirmed that the intracellular calcium mobilization of HDP subpopulations was significantly higher than both subpopulations of IDP and LDP. Conclusions: HDP subgroup platelets are highly functional and most sensitive to aggregating agents.