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研究了来自不同地理区域的５１个香菇野生菌株的线粒体ＤＮＡ（ｍｔＤＮＡ）的限制性片段长度多态性（ＲＦＬＰｓ），其中３８株来自日本，７株来自巴布新几内亚，４株来自新西兰，１株来自婆罗州，１株来自泰国。把它们的ｍｔＤＮＡｓ用限制酶ＥｃｏＲＩ、ＢａｍＨＩ消化，分别产生２４、１２种ＲＦＬＰ型。经比较，５１个野生香菇菌株的ｍｔＤＮＡ有２８种表型。以共迁移限制性片段为基础，计算表型对的相似矩阵，采用ＵＰＧＭＡ聚类，Ｆｉｔｃｈ－Ｍａｒｇｏｌｉａｓｈ法分析，结果同时表明香菇ｍｔＤＮＡ表型分成５群，与各自的地理区域相对应。本研究所得到的系统发育关系证实先前所作的同工酶分析结果，在明香菇存在几个种内组，它们的线粒体和核基因组存在遗传差异。
The restriction fragment length polymorphisms (RFLPs) of mitochondrial DNA (mtDNA) from 51 wild mushroom strains from different geographic regions were studied, of which 38 were from Japan, 7 were from Papua New Guinea, 4 were from New Zealand, 1 The strain came from Borneo, one from Thailand. Their mtDNAs were digested with restriction enzymes EcoRI and BamHI to generate 24 and 12 RFLP types, respectively. By comparison, 51 mtDNA strains of wild shiitake mushrooms have 28 phenotypes. The similarity matrix of the phenotypic pairs was calculated on the basis of the co-migrating restriction fragments. UPGMA clustering and Fitch-Margoliash analysis were performed. The results also showed that the mtDNA phenotype of the shiitake mushrooms was divided into five groups, corresponding to their geographical regions. The phylogenetic relationship obtained in this study confirms the results of the isoenzyme analysis previously conducted. There are several species within the Mushrooms and there are genetic differences between their mitochondria and nuclear genomes.