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目的探讨PTEN蛋白及其作用底物P-Tyr在不同胶质瘤中的表达差异和临床意义。方法对69例胶质瘤(髓母细胞瘤34例;间变型或胶质母细胞瘤10例;室管膜瘤10例;纤维型或毛细胞型低级别星形细胞瘤15例;)进行PTEN和P-Tyr免疫组化染色,评价不同类型胶质瘤PTEN阳性表达率,半定量测定其平均染色强度IS(intensity score)。结果髓母细胞瘤、恶性胶质瘤、室管膜瘤和低级别星形细胞瘤的PTEN阳性表达率分别为:23.5%、20%、30%、80%;PTEN IS均值分别为(0.59±0.43)、(0.47±0.32)、(0.39±0.32)、(1.17±0.52);P-Tyr的阳性表达率分别为91.2%、80%、90%、73.3%;P-Tyr IS的均值为(1.92±0.79)、(1.41±0.44)、(1.72±0.69)、(1.16±0.48)。髓母细胞瘤、恶性胶质瘤和低级别星形细胞瘤在PTEN阳性表达率和染色强度上存在明显差异(P<0.01),所有胶质瘤的PTEN和P-Tyr染色强度IS呈负相关。结论PTEN表达缺失与肿瘤的生物学行为密切相关,系肿瘤恶性转化和肿瘤增殖活性增加的主要原因;PTEN与P-Tyr的表达呈负相关说明,PTEN具有蛋白酪氨酸磷酸酯酶活性,并起抑制肿瘤效应。
Objective To investigate the difference and clinical significance of PTEN protein and its role as substrate P-Tyr in different gliomas. Methods Sixty-nine gliomas (34 cases of medulloblastoma, 10 cases of metaplasia or glioblastoma, 10 cases of ependymoma, 15 cases of fibroid or hair cell type low-grade astrocytoma) PTEN and P-Tyr immunohistochemical staining to evaluate the different types of glioma PTEN positive expression rate, semi-quantitative determination of the average staining intensity IS (intensity score). Results The positive rates of PTEN in medulloblastoma, glioblastoma, ependymoma and low-grade astrocytoma were 23.5%, 20%, 30% and 80%, respectively. The average PTEN IS was (0.59 ± P <0.01). The positive expression rates of P-Tyr were 91.2%, 80%, 90% and 73.3%, respectively. The mean of P-Tyr IS was (0.47 ± 0.32), (0.39 ± 0.32) and (1.17 ± 0.52) 1.92 ± 0.79), (1.41 ± 0.44), (1.72 ± 0.69), (1.16 ± 0.48) respectively. Medulloblastoma, glioblastoma and low-grade astrocytoma in PTEN positive expression rate and staining intensity were significantly different (P <0.01), all gliomas PTEN and P-Tyr staining intensity IS was negatively correlated . Conclusions The loss of PTEN expression is closely related to the biological behavior of the tumor, which is the main reason for the malignant transformation and tumor proliferation. The negative correlation between the expression of PTEN and P-Tyr indicates that PTEN has the activity of protein tyrosine phosphatase From the inhibition of tumor effect.