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目的:研究内关穴位注射香丹注射液预处理对大鼠心肌缺血炎性因子白介素10(IL-10)、白介素18(IL-18)的影响。方法:以SD大鼠为受试对象,穴位注射香丹注射液预处理14天,然后腹腔皮下注射异丙肾上腺素(ISO)造成心肌缺血模型,采用酶联免疫吸附法(ELISA)检测IL-10、IL-18。结果:(1)IL-10在心肌缺血后含量增多,内关组、药物对照组及曲池组预处理后可以升高其含量,内关组的这一作用明显优于曲池组及药物对照组。(2)IL-18在心肌缺血后表达增多,预处理后内关组可以降低其表达,曲池组及药物对照组对其改善不明显,内关组的这一作用明显优于曲池组及药物对照组。(3)IL-18/IL-10值的比较:内关组、药物对照组、曲池组与模型组比较有显著性差异,内关组与曲池组、药物对照组比较有显著性差异,曲池组与药物对照组比较则无明显差别。结论:内关穴位注射香丹注射液预处理对缺血大鼠心肌具有保护作用。
Objective: To investigate the effect of injection of Xiangdan injection into Neiguan injection on the expression of interleukin 10 (IL-10), interleukin 18 (IL-18) in rats with myocardial ischemia. Methods: Sprague-Dawley rats were used as experimental subjects, acupoint injection of Xiangdan injection for 14 days and then intraperitoneal injection of isoproterenol (ISO) to induce myocardial ischemia. ELISA was used to detect IL -10, IL-18. Results: (1) The content of IL-10 increased after myocardial ischemia, and the content of IL-10 increased after pretreatment in Neiguan group, drug control group and Quchi group. The effect of NeiGuan group was better than Quchi group and Drug control group. (2) The expression of IL-18 increased after myocardial ischemia, and the expression of IL-18 decreased after preconditioning, while Quchi group and drug control group had no significant improvement. Neiguan group was better than Quchi Group and drug control group. (3) The comparison of IL-18 / IL-10 values: There were significant differences between Neiguan group, drug control group and Quchi group and model group, there was significant difference between Neiguan group and Quchi group and drug control group , Quchi group and drug control group no significant difference. Conclusion: Neiguan injection of Xiangdan injection pretreatment has a protective effect on myocardial ischemia in rats.