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我们对四种不同病因所致的精神发育迟滞儿童血清蛋白进行了等电点聚焦(IEF)电泳的检测,现报道如下。材料与方法 1.研究对象:选择住院精神发育迟滞儿童7例,男4例,女3例,年龄5至8岁,其中先天愚型2例;精神发育迟滞伴发癫痫2例;精神发育迟滞有产伤史2例;精神发育迟滞有新生儿期破伤风感染史1例;7例患儿智商均低于49,无贫血、营养不良和肝肾等疾病。同时选择智力正常的7岁儿童男女各1名作对照。 2.血清制备:抽取手臂静脉血液1ml,以2,500转/分离心沉淀10分钟,吸取上清液备用。 3.等电点聚焦电泳:采~1用LKB—2117型
We examined four different etiology of mental retardation in children with serum protein isoelectric focusing (IEF) electrophoresis, are reported below. MATERIALS AND METHODS 1. Subjects: Seven children with in-hospital mental retardation, 4 males and 3 females, aged 5 to 8 years, including 2 cases of Down’s syndrome; 2 children with mental retardation and epilepsy, 2 children with mental retardation There are 2 cases of history of birth injury; mental retardation has a neonatal tetanus infection in 1 case; 7 cases of children with IQ lower than 49, no anemia, malnutrition and liver and kidney disease. At the same time choose a normal mental 7-year-old children, a male and female as a control. 2. Serum Preparation: Extraction of arm vein blood 1ml, 2,500 rev / centrifugation precipitation 10 minutes, the supernatant was as spare. 3. Isoelectric focusing electrophoresis: mining ~ 1 LKB-2117 type