论文部分内容阅读
为了建立葛花中大豆苷、鸢尾苷和染料木素3种异黄酮成分的HPLC测定方法,试验采用色谱柱为Eclipse XDB-C18(4.6 mm×150 mm,5μm),流动相为乙腈-1‰磷酸水溶液,梯度洗脱(0~20 min,13%乙腈;20~30 min,27%乙腈;30~40 min,32%乙腈;40~50 min,36%乙腈;50~60 min,43%乙腈),流速为0.8 m L/min,检测波长为264 nm,柱温为30℃的HPLC方法进行检测。结果表明:大豆苷、鸢尾苷和染料木素的线性范围分别为0.126~5.040 mg/m L(R2=0.999 7),0.096~3.840 mg/m L(R2=0.999 6)和0.034~1.360 mg/m L(R2=0.999 9);平均回收率分别为99.22%(RSD=1.11%)、103.89%(RSD=1.89%)和99.93%(RSD=1.53%)。说明该方法能够将葛花中多种异黄酮类成分分离,准确可靠,专属性强,结果稳定,可用于葛花药材的质量控制。
In order to establish an HPLC method for the determination of daidzin, tectorigen and genistein in Isatidis Hedysari, Eclipse XDB-C18 (4.6 mm × 150 mm, 5 μm) was used as the chromatographic column. The mobile phase consisted of acetonitrile-1 ‰ Aqueous solution of phosphoric acid and gradient elution (0-20 min, 13% acetonitrile; 20-30 min, 27% acetonitrile; 30-40 min, 32% acetonitrile; 40-50 min, 36% acetonitrile; Acetonitrile) at a flow rate of 0.8 m L / min and a detection wavelength of 264 nm at a column temperature of 30 ° C. The results showed that the linear ranges of daidzin, tectorigen and genistein were 0.126 ~ 5.040 mg / m L (R2 = 0.999 7), 0.096 ~ 3.840 mg / m L (R2 = 0.999 6) and 0.034 ~ 1.360 mg / The average recoveries were 99.22% (RSD = 1.11%), 103.89% (RSD = 1.89%) and 99.93% (RSD = 1.53%), respectively. The results showed that this method could separate a variety of isoflavones in Pueraria lobata, which was accurate and reliable, strong specificity and stable result, which could be used for the quality control of Gehua medicinal materials.