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目的初步探讨液态氟碳(perfluorooctyl-bromide,PFOB)纳米粒对蛛网膜下腔出血后早期脑损的保护作用及其与低氧诱导因子1α(HIF-1α)的关系。方法健康雄性SD大鼠100只分为5个组:假手术组(Sham)、蛛血组(SAH)、蛛血+安慰剂组(SAH+vehicle)、蛛血+5 g/kg PFOB组(SAH+5 g/kg PFOB)和蛛血+10 g/kg PFOB组(SAH+10 g/kg PFOB),每组20只。采用颈内动脉穿刺法制作SAH模型。造模后24 h对每组大鼠进行神经行为功能缺损评分,检测脑含水量和血脑屏障(BBB)通透性,末端脱氧核苷酸介导的X-d UTP缺口末端标记法(TUNEL)检测神经元凋亡,免疫组化和Western blot检测HIF-1α蛋白表达情况。结果 PFOB纳米粒能够显著降低早期脑损伤,包括改善神经功能缺失、减轻脑水肿、降低血脑屏障通透性和减少神经元凋亡,且SAH+10 g/kg PFOB组降低效果明显高于SAH+5 g/kg PFOB组(P<0.05)。PFOB在降低早期脑损伤的同时能够显著抑制HIF-1α蛋白表达,且SAH+10 g/kg PFOB组抑制效果明显高于SAH+5 g/kg PFOB组(P<0.05)。结论液态氟碳纳米粒对蛛网膜下腔出血后早期脑损伤的保护作用可能与抑制HIF-1α蛋白表达有关。
Objective To investigate the protective effect of perfluoroctyl-bromide (PFOB) nanoparticles on early brain injury after subarachnoid hemorrhage and its relationship with hypoxia inducible factor 1α (HIF-1α). Methods 100 healthy male SD rats were divided into five groups: sham group, SAH group, SAH + vehicle group and spleen group + 5 g / kg PFOB group SAH + 5 g / kg PFOB) and spider blood + 10 g / kg PFOB group (SAH + 10 g / kg PFOB), 20 in each. SAH model was made by carotid artery puncture. Neurological deficits in each group were measured 24 h after model establishment. Brain water content and permeability of the blood-brain barrier (BBB) were measured. Terminal deoxynucleotide-mediated Xd-UTP nick end labeling (TUNEL) Neuronal apoptosis, immunohistochemistry and Western blot were used to detect the protein expression of HIF-1α. Results PFOB nanoparticles could significantly reduce the early brain injury, including improving neurological deficit, relieving cerebral edema, decreasing blood-brain barrier permeability and decreasing neuronal apoptosis. The effect of PFOB nanoparticle in SAH + 10 g / kg PFOB group was significantly lower than that of SAH +5 g / kg PFOB group (P <0.05). PFOB could significantly inhibit the expression of HIF-1α protein in early brain injury, and the inhibitory effect of PFOB in SAH + 10 g / kg PFOB group was significantly higher than that in SAH + 5 g / kg PFOB group (P <0.05). Conclusion The protective effect of liquid fluorocarbon nanoparticles on early brain injury after subarachnoid hemorrhage may be related to the inhibition of HIF-1α protein expression.