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乙胺嘧啶与周效磺胺合剂(法西达)普遍用于治疗抗氯喹恶性疟,而许多地区的恶性疟原虫对法西达产生抗性。过去体外测定乙胺嘧啶反应的M RPMI-1640标准培养液内含有高浓度的,对周效磺胺有拮抗作用的对氨基苯甲酸(PABA)和叶酸。本文介绍一种适合于现场评价恶性疟原虫对法西达敏感性的体外测试法,并比较了疟原虫在体内和体外药物反应的关系。1981年1～3月在肯尼亚Malindi超高度疟区选择单纯恶性疟感染,无性体原虫数≥50/300白细胞,2周内无服药史的35名6～7岁儿童为研究对象。
Pyrimethamine and psoxamide (faxacid) are commonly used to treat chloroquine-resistant P. falciparum, whereas P. falciparum in many areas is resistant to faxidac. Previous MTPMI-1640 standard media for in vitro pyrimethamine reactions contained high concentrations of p-aminobenzoic acid (PABA) and folic acid, which antagonized psoralen. In this paper, we present an in vitro assay suitable for the field-based evaluation of the susceptibility of Plasmodium falciparum to faxidis and compare the drug response of Plasmodium in vivo and in vitro. From January to March 1981, 35 children aged 6 to 7 years old with no falciparum malaria infection, no more than 50/300 leukoplakia and no medication history within 2 weeks were enrolled in this study.