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目的建立雪抑乐中主要有效成分积雪草苷和羟基积雪草苷的含量测定方法。方法采用HPLC-ELSD法,Kromasil ODS C18色谱柱(150 mm×4.6 mm,5μm),柱温30℃,流动相为甲醇-水(48∶52),流速0.8 mL/min,ELSD检测器漂移管温度90℃,空气流速2.1 L/min。结果积雪草苷和羟基积雪草苷线性范围分别为1.55~12.40μg和1.68~13.40μg;积雪草苷回收率为98.35%,RSD为2.33%;羟基积雪草苷回收率99.23%,RSD 2.16%。结论所建立的方法快速、准确,可作为雪抑乐的质量控制方法。
Objective To establish a method for determining the content of asiaticoside and madecassoside, which are the main active ingredients in Xuexue. METHODS HPLC-ELSD method was used on a Kromasil ODS C18 column (150 mm×4.6 mm, 5 μm). The column temperature was 30°C. The mobile phase was methanol-water (48:52). The flow rate was 0.8 mL/min. The ELSD detector drift tube was used. Temperature 90°C, air flow rate 2.1 L/min. Results The linear ranges of asiaticoside and madecassoside were 1.55~12.40μg and 1.68~13.40μg, respectively. The recovery rate of asiaticoside was 98.35%, the RSD was 2.33%, and the recovery rate of madecassoside was 99.23%. RSD 2.16%. Conclusion The established method is rapid and accurate and can be used as a method of quality control for snow suppression.