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目的:观察表没食子儿茶素没食子酸酯(EGCG)对APP/PS1转基因小鼠海马胰岛素受体底物-1(IRS-1)磷酸化的作用。方法:12月龄雌性APP/PS1小鼠随机分为模型组(Tg)、EGCG低剂量组(Tg/EGCG-L)、高剂量组(Tg/EGCG-H),同月龄雌性C57BL/6J小鼠作为对照组(NT)。采用Western blot和免疫组织化学方法检测各组小鼠海马IRS-1pSer636的表达。结果:与对照组比较,模型组小鼠海马IRS-1pSer636表达明显升高(P<0.05),EGCG各治疗组IRS-1pSer636表达较模型组降低(P<0.05)。结论:EGCG可以减轻APP/PS1小鼠海马胰岛素抵抗,其机制可能部分与减轻IRS-1在丝氨酸636位点的磷酸化水平有关。
AIM: To observe the effect of epigallocatechin gallate (EGCG) on phosphorylation of insulin receptor substrate-1 (IRS-1) in hippocampus of APP / PS1 transgenic mice. Methods: 12-month old female APP / PS1 mice were randomly divided into three groups: model group (Tg), EGCG low dose group (Tg / EGCG-L), high dose group Rats served as control group (NT). Western blot and immunohistochemistry were used to detect the expression of IRS-1pSer636 in hippocampus of each group. Results: Compared with the control group, the expression of IRS-1pSer636 in model group was significantly increased (P <0.05). The expression of IRS-1pSer636 in EGCG treatment group was lower than that in model group (P <0.05). CONCLUSION: EGCG can attenuate hippocampal insulin resistance in APP / PS1 mice. The mechanism may be partly related to the decrease of phosphorylation of IRS-1 at serine 636.