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目的:探讨血府逐瘀汤对局灶性脑缺血损伤大鼠的保护作用及其可能的作用机制。方法:采用大鼠永久性大脑中动脉阻塞(MCAO)方法建立局灶性脑缺血模型,灌胃给予血府逐瘀汤(5,10,20 g·kg-1)进行干预,通过神经症状评分评价大鼠行为学的改变,TTC染色法观察脑梗死范围,紫外分光光度法检测缺血脑组织丙二醛(MDA)、一氧化氮(NO)含量及超氧化物歧化酶(SOD)活性,实时荧光定量PCR技术检测缺血大脑皮层凋亡相关基因Bax、Bcl-2、Caspase-3的mRNA表达。结果:血府逐瘀汤可显著改善MCAO大鼠神经症状,减少脑梗死体积,降低缺血脑组织中MDA、NO含量,提高SOD活性,同时下调缺血大脑皮层Bax、Caspase-3的mRNA表达,上调Bcl-2 mRNA表达。结论:血府逐瘀汤对局灶性脑缺血大鼠脑损伤有保护作用,其作用机制可能与其抗氧化及抑制凋亡有关。
Objective: To investigate the protective effect of Xuefu Zhuyu Decoction on focal cerebral ischemia injury in rats and its possible mechanism. Methods: The model of focal cerebral ischemia was established by permanent middle cerebral artery occlusion (MCAO) in rats. After administration of Xuefu Zhuyu Decoction (5, 10, 20 g · kg-1) The scores of rats were evaluated by behavioral changes. The extent of cerebral infarction was observed by TTC staining. The contents of malondialdehyde (MDA), nitric oxide (NO) and superoxide dismutase (SOD) in ischemic brain tissue were detected by UV spectrophotometry. Real-time fluorescence quantitative PCR was used to detect the mRNA expression of Bax, Bcl-2 and Caspase-3 in ischemic cerebral cortex. Results: Xuefu Zhuyu Decoction could significantly improve the neurological symptoms, reduce the volume of cerebral infarction, decrease the content of MDA and NO in ischemic brain tissue, increase the activity of SOD and down-regulate the mRNA expression of Bax and Caspase-3 in ischemic cerebral cortex , Up-regulate Bcl-2 mRNA expression. Conclusion: Xuefu Zhuyu Decoction has a protective effect on brain injury induced by focal cerebral ischemia in rats. Its mechanism may be related to its anti-oxidation and anti-apoptotic effects.