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目的 :了解严重急性呼吸道综合征 (SARS)特异性IgM和IgG抗体的变化规律。方法 :采用间接酶联免疫吸附试验 (ELISA)检测 2 0例SARS患者系列血清中特异性IgM和IgG抗体 ,系列血清包括患者发病后 1周 ,2周 ,3周 ,4周 ,8周 ,12周所采集的样本。结果 :2 0例患者发病后第 1周IgM和IgG抗体均为阴性 ;第 2周时 16例IgM抗体阳性 ,17例IgG抗体阳性 ;第 3周后所有患者IgG抗体阳性并持续至第 12周 ,而IgM抗体阳性患者逐渐减少 ,至第 12周时所有患者均为阴性。结论 :SARS特异性IgM抗体消失较早 ,其存在是近期感染的标志 ;IgG抗体的持续存在可能是获得病后免疫力的标志。
OBJECTIVE: To investigate the changes of specific IgM and IgG antibodies against severe acute respiratory syndrome (SARS). Methods: The specific serum IgM and IgG in serum of 20 patients with SARS were detected by indirect enzyme-linked immunosorbent assay (ELISA) .Serial sera including 1 week, 2 weeks, 3 weeks, 4 weeks, 8 weeks, 12 Weekly collected samples. Results: IgM and IgG antibodies were negative in the first week after the onset of disease in 20 patients. 16 cases were positive for IgM antibody and 17 cases were positive for IgG antibody in the second week. All patients were positive for IgG antibody after the third week and continued till the twelfth week , And IgM antibody-positive patients gradually decreased, by the 12th week, all patients were negative. CONCLUSIONS: SARS-specific IgM antibodies disappear earlier and their presence is a marker of recent infection. The persistence of IgG antibodies may be a marker of acquired immunity.