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提出了曙红Y-Fe2+-H2O2化学发光新体系,基于酸性条件下黄豆苷元对新体系的发光强度有明显的抑制作用,建立了流动注射化学发光抑制法测定黄豆苷元含量及其抗氧化活性的新方法。考察了影响化学发光强度的因素。在最佳条件下,方法的线性范围为8.0×10-8~3.0×10-6 mol/L,检出限为9.0×10-9 mol/L-1。对1.0×10-6 mol/L-1的黄豆苷元进行11次平行测定,其相对标准偏差为4.6%。此法用于黄豆苷元片剂含量测定,结果令人满意。采用二甲亚砜和甘露醇作为参照物对其羟自由基清除率进行测评,结果表明,三种物质抗氧化活性大小顺序为黄豆苷元>二甲亚砜>甘露醇。
A new chemiluminescence system based on eosin Y-Fe2 + -H2O2 was proposed. Based on the acidic conditions, daidzein obviously inhibited the luminescence intensity of the new system. Flow injection chemiluminescence inhibition was used to determine the content of daidzein and its antioxidant activity New method of activity. The factors affecting the chemiluminescence intensity were examined. Under the optimum conditions, the linear range of the method was 8.0 × 10-8 ~ 3.0 × 10-6 mol / L with the detection limit of 9.0 × 10-9 mol / L-1. The parallel determination of daidzein at 1.0 × 10-6 mol / L-1 for 11 replicates showed a relative standard deviation of 4.6%. This method is used for the determination of daidzein tablets, the results are satisfactory. Dimethyl sulfoxide and mannitol were used as reference to evaluate their hydroxyl radical scavenging activity. The results showed that the order of antioxidant activity of the three substances was daidzein> dimethyl sulfoxide> mannitol.