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大鼠吸入H_2S 200ppm3h中毒,测定了中毒后3,12,24,48.72和96h血清及防线粒体-溶酶体、细胞核、细胞浆及微粒体中LDH、ACP、CPK及Na~+-K~+-ATP酶活性的变化。结果表明,H_2S吸入中毒后亚细胞水平的肺损伤具有发生快、维持时间长等特点,中毒后3h即有溶酶体线粒体破裂、细胞能量代谢抑制、细胞破裂及标记酶入血,12h亚细胞损伤达高峰,随后渐恢复,72h恢复至正常水平,血清酶活性变化与亚细胞水平的变化高度相关,提示血清酶的变化源于肺损伤,因此,测定血清酶的变化能较好地反映亚细胞水平的肺损伤。
Inhalation of H_2S 200ppm3h poisoned rats, the levels of LDH, ACP, CPK and Na ~ + -K ~ + in serum and mitochondria-lysosomes, nuclei, cytoplasm and microsome of 3, 12, 24, 48.72 and 96h after poisoning were measured. -ATP enzyme activity changes. The results showed that submerged lung injury induced by H 2 S inhalation was rapid and maintained for a long time. Mitochondrial lysosomal rupture, inhibition of cellular energy metabolism, cell rupture and labeling of enzyme into blood were observed at 3h after poisoning. 12h subcellular The damage reached the peak, then gradually recovered, and recovered to normal level at 72h. The change of serum enzyme activity was highly correlated with the change of subcellular level, suggesting that the change of serum enzyme originated from lung injury. Therefore, Lung injury at the cellular level.