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增殖细胞核抗原(PCNA)是DNA聚合酶的辅助蛋白,其合成与细胞增殖周期有关。G_1晚期开始表达逐渐增加,S期达高峰,G_2期、M期明显下降,能客观反应细胞增殖状况。本研究应用免疫组化LSAB法探讨喉癌组织中PCNA表达程度与其组织学分级和颈淋巴结转移的相关性,以期为喉癌临床综合治疗提供实验依据。采用本院1993～1996年36例原发喉鳞癌手术标本,术前均未经放疗或化疗,其中颈淋巴结转移11例,低分化鳞癌10例,中分化鳞癌11例,高分化鳞癌15
Proliferating cell nuclear antigen (PCNA) is an accessory protein of DNA polymerase, whose synthesis is related to cell cycle. The expression of G_1 gradually increased at the beginning of G_1 phase, reached its peak in S phase, and decreased significantly in G_2 phase and M phase, which could objectively reflect the cell proliferation status. In this study, the immunohistochemical LSAB method was used to investigate the correlation between the expression of PCNA and the histological grade and cervical lymph node metastasis in laryngeal carcinoma, in order to provide experimental evidence for clinical treatment of laryngeal cancer. Using our hospital from 1993 to 1996, 36 cases of primary laryngeal squamous cell carcinoma specimens were not radiotherapy or chemotherapy before surgery, including cervical lymph node metastasis in 11 cases, poorly differentiated squamous cell carcinoma in 10 cases, moderately differentiated squamous cell carcinoma in 11 cases, Cancer 15