论文部分内容阅读
本文研究目的为:(1)确定孕酮是否能拮抗雌激素引起的加速卵的运输作用。(2)检查雌激素刺激和加速卵运输之间输卵管受体水平的变化。(3)确定应用孕酮能否改变由输卵管雌激素诱导的雌激素受体水平的变化。(4)证实孕酮对卵的运输和对输卵管雌激素受体的动力学影响系经孕酮受体介导。当评价此点用 RU486阻断孕酮受体。实验采用成年雌性 Spraque-Dawley 大鼠,通过阴道细胞涂片确定动情周期变化。在动情前期与有生育力的雄鼠合笼,次日如在阴道发现精子定为妊娠第一天。一组在妊娠第一天注射雌二醇(E_2)。二组注射雌二醇和孕酮(E_2·P)。三组同上,但为阻断孕酮受体,于皮下埋置 RU486渗透泵对照组
The purpose of this study is: (1) to determine whether progesterone can antagonize estrogen-induced accelerated transport of eggs. (2) Check the change of tubal receptor level between estrogen stimulation and accelerated egg transport. (3) Determine whether the use of progesterone can alter the estrogen-induced estrogen receptor level changes in oviduct. (4) The prokinetic effect of progesterone on egg transport and on the estrogen receptor of the fallopian tube is mediated by the progesterone receptor. When evaluated, RU486 blocked the progesterone receptor. Adult female Spraque-Dawley rats were used in the experiment to determine estrous cycle changes by vaginal smears. In pre-estrus and fertile male cage, the next day as found in the vagina sperm as the first day of pregnancy. One group received estradiol (E2) on the first day of gestation. Two groups were injected with estradiol and progesterone (E_2 · P). The three groups were the same as above, but to block the progesterone receptor, RU486 osmotic pump control group was subcutaneously embedded