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目的通过比较维吾尔族宫颈癌与正常宫颈中NF-κB和HIF-la表达的差异,了解维吾尔族宫颈癌与NF-κB和HIF-la的关系及意义。方法采用含20 000条Oligo DNA的人类全基因组寡核苷酸芯片,对5例维吾尔族宫颈癌与正常宫颈成组检测差异表达基因,通过MAS分析得到两组间差异表达基因:NF-κB和HIF-la,并采用RT-PCR及免疫组织化学(免疫组化)验证芯片检测其mRNA和蛋白的表达。结果 12例宫颈癌组织中NF-κB、HIF-la mRNA的相对表达量均显著高于正常宫颈组,免疫组化结果显示:1NF-κB蛋白与HIF-la蛋白在宫颈癌组和正常宫颈组中表达差异有统计学意义(t=11.393、11.809,P<0.05);2宫颈癌组织中NF-κB、HIF-la蛋白阳性表达与年龄无关(χ~2=2.918、2.920,P>0.05),与病理分化成度及临床分期相关,差异有统计学意义(χ~2=11.565、14.799;18.026、17.432,P<0.05);3NF-κB和HIF-la在宫颈癌组中表达呈正相关(r=0.368,P<0.05)。结论 NF-κB和HIF-la蛋白的阳性表达随着宫颈癌的分化程度和临床分期的升高逐步升高,在宫颈癌组织中NF-κB表达量的升高与HIF-la表达量升高正相关,初步证明了NF-κB可能通过激活PI3K/AKt途径上调HIF-la的表达,促进了宫颈癌的发生、发展、浸润和转移。
Objective To investigate the relationship between Uygur cervical cancer and NF-κB and HIF-la by comparing the difference of expression of NF-κB and HIF-la between Uigur cervical cancer and normal cervix. Methods Human genome-wide oligonucleotide microarray containing 20 000 Oligo DNAs was used to detect differentially expressed genes in 5 cases of Uigur cervical cancer and normal cervix. The gene expression of NF-κB and NF- HIF-la was detected by RT-PCR and immunohistochemistry (IHC). Results The relative expression of NF-κB and HIF-1a mRNA in cervical cancer tissues was significantly higher than that in normal cervical tissues. The results of immunohistochemistry showed that the expression of NF-κB and HIF-la in cervical cancer group and normal cervical group (T = 11.393, 11.809, P <0.05). The positive expressions of NF-κB and HIF-la in cervical cancer tissues were not related to age (χ ~ 2 = 2.918,2.920, P> 0.05) (Χ ~ 2 = 11.565,14.799; 18.026,17.432, P <0.05). There was a positive correlation between the expression of 3NF-κB and HIF-la in cervical cancer r = 0.368, P <0.05). Conclusion The positive expression of NF-κB and HIF-la protein gradually increases with the differentiation and clinical stage of cervical cancer. The expression of NF-κB and the expression of HIF-la are up-regulated in cervical cancer It is preliminarily proved that NF-κB may up-regulate the expression of HIF-1a by activating the PI3K / AKt pathway and promote the occurrence, development, invasion and metastasis of cervical cancer.