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本实验从人恒牙内分离出牙髓细胞，进行组织块培养。培养３天，见组织块边缘有梭形细胞游出，随着培养天数的增多，游出的细胞也逐渐增多。来源于相邻组织块的细胞团逐渐靠近、接触；培养２０天时，细胞长满瓶底，铺成单层。按１：３的比例进行传代，平均６天传代一次，观察细胞的形态、排列及增殖情况。从细胞生长曲线中可以看到：接种后第１天，细胞基本无增长；第２～５天，细胞增长旺盛；第６天，细胞生长又趋于缓慢；第７天时，增长已近停止。本实验结果表明人牙髓细胞体外培养的可行性。
In this experiment, dental pulp cells were isolated from human permanent teeth, and tissue culture was performed. After culturing for 3 days, spindle cells migrated out of the edge of the tissue mass. As the number of days of culture increased, the number of exiting cells also gradually increased. Cells from adjacent tissue blocks gradually close contact, contact; cultured 20 days, the bottom of the bottle covered with a single layer. According to the ratio of 1: 3, the cells were passaged on the average of 6 days to observe the morphology, arrangement and proliferation of the cells. From the cell growth curve we can see: on the first day after inoculation, the cells showed almost no growth; on the second to fifth days, the cells grew vigorously; on the sixth day, the cell growth tended to be slow; on the seventh day, the growth had almost stopped. The experimental results show that human dental pulp cells in vitro culture feasibility.